Application

Research Sub CategoryNuclear Receptors

Research CategoryEpigenetics & Nuclear Function

This Anti-Acetylated Nucleophosmin Antibody, clone 31M1 is validated for use in Immunohistochemistry (Paraffin) and Western Blotting and Immunocytochemistry for the detection of Acetylated Nucleophosmin.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Acetylated Nucleophosmin in kidney tissue.
Western Blotting Analysis: A representative lot detected Acetylated Nucleophosmin in acetylated and non-acetylated (mock acetylation control) forms of recombinant NPM1 and whole cell extract of HEK293T cell line (Prof. T K Kundu, Nehru Centre for Advanced Scientific Research, JNCASR).
Immunocytochemistry Analysis: A representative lot detected Acetylated Nucleophosmin in HEK293 cells (Prof. T K Kundu, Nehru Centre for Advanced Scientific Research, JNCASR).
Immunohistochemistry Analysis: A representative lot detected Acetylated Nucleophosmin in paraffin embedded sections of human oral cancer tissue and adjacent normal tissue (Prof. T K Kundu, Nehru Centre for Advanced Scientific Research, JNCASR).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Nucleophosmin/B23 is a major nucleolar phosphoprotein that is mainly located within the nucleolar granular compartment, where preribosomes are assembled. Nucleophosmin/B23 is a multifunctional protein that is overexpressed in actively proliferating cells and cancer cells. Antibodies against B23 might be useful for recognition of human peripheral lymphocytes at early stages of their activation for proliferation, preceding the S-phase. By parallel immunolabeling with the anti-Ki-67 antibody, it was shown that the early changes of B23 amount and localization occurred before an appearance of Ki-67 protein, a well-known marker of proliferating cells.

Immunogen

Linear peptide corresponding to human Acetylated Nucleophosmin.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Immunohistochemistry in human kidney tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Acetylated Nucleophosmin in kidney tissue.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

33 kDa calculated